2BPC157 The BPC157 peptide synthesis process of the pilot scale of embodiment 2 12020g(Loadding1.0mmol/g)1TBTU/HOBt-24gHPLC751C 18 (Kromasil-C 18 30mm*250mm)13.6gHPLC98 Referring to the specific process of Example 1, the difference is that all reagents are directly amplified 20 times, that is, 20 g of Wang's resin (Loadding: 1.0 mmol/g) is weighed for reaction, and the TBTU/HOBt reaction of Example 1 is also used to prepare the resin - Peptide conjugates, cleaved to prepare crude peptides, a total of 24 g of crude products were obtained, with a purity of 75% as determined by HPLC
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To use it, you must reconstitute itreintroduce a liquid to turn it into a usable solution
Wipe both rubber stoppers with a fresh alcohol pad
Then, the sample tubes were centrifuged at 16,000 g and the supernatants were discarded after each washing step
The consensus generally favors whichever method the researcher is more comfortable and precise with